Isolation and characterization of microsatellites in the endoparasitic ichneumonid wasp carrying a polydnavirus Hyposoter didymator
نویسندگان
چکیده
منابع مشابه
Co-replication of a reovirus and a polydnavirus in the ichneumonid parasitoid Hyposoter exiguae.
A recently established colony of the ichneumonid parasitoid, Hyposoter exiguae, was found to carry both a reovirus (HeRV) and a polydnavirus (HePDV). Morphogenesis of these viruses was observed in all cells comprising the ovarian calyx epithelium, apparently without detrimental effect to the parasitoid. While polydnavirus replication in H. exiguae was restricted to the calyx region, HeRV was de...
متن کاملPersistent expression of a newly characterized Hyposoter didymator polydnavirus gene in long-term infected lepidopteran cell lines.
An Hyposoter didymator ichnovirus (HdIV) gene was stably maintained and efficiently transcribed in lepidopteran cell lines more than 3 years after HdIV infection. This K-gene had two introns and the fully spliced cDNA, named K19, comprised a short open reading frame and a long 3'-untranslated region with 13 imperfectly repeated sequences (44 to 102 nt). Transcripts related to the K-gene were de...
متن کاملAnalysis of Virion Structural Components Reveals Vestiges of the Ancestral Ichnovirus Genome
Many thousands of endoparasitic wasp species are known to inject polydnavirus (PDV) particles into their caterpillar host during oviposition, causing immune and developmental dysfunctions that benefit the wasp larva. PDVs associated with braconid and ichneumonid wasps, bracoviruses and ichnoviruses respectively, both deliver multiple circular dsDNA molecules to the caterpillar. These molecules ...
متن کاملWasp Polydnavirus Polymorphism
In this paper, we analyze the polymorphisms of parasitoid wasp polydnavirus. The polydnavius genome is normally dormant within the genome of the wasp, but is converted into particles that are injected into the infected host along with the wasp eggs. These particles contain genes which inhibit the immune system of the infected host, and are necessary for the eggs to mature. Within a population, ...
متن کاملIsolation and characterization of microsatellites in lane
Polymerase chain reaction primer pairs for a total of 25 nuclear-encoded microsatellites (loci) were developed from genomic DNA libraries of lane snapper ( Lutjanus synagris ), mutton snapper ( Lutjanus analis ), and yellowtail snapper ( Ocyurus chrysurus ). The microsatellites include 24 perfect (21 dinucleotide and three trinucleotide) and one imperfect (combination tetranucleotide/tetranucle...
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ژورنال
عنوان ژورنال: Conservation Genetics Resources
سال: 2013
ISSN: 1877-7252,1877-7260
DOI: 10.1007/s12686-013-0036-0